ALL PUBLICATIONS

Pedro N. Leão*, Teresa P. Martins, Kathleen Abt, João P. A. Reis, Sandra Figueiredo, Raquel Castelo-Branco and Sara Freitas (2023). Incorporation and modification of fatty acids in cyanobacterial natural products biosynthesis. Chem. Commun., Advance Article doi: doi.org/10.1039/D3CC00136A

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Amaranta Kahn, Paulo Oliveira, Marine Cuau, Pedro Leão* (2023) Incorporation, fate, and turnover of free fatty acids in cyanobacteria FEMS Microbiology Reviews, Volume 47, Issue 2, March 2023, fuad015, doi: 10.1093/femsre/fuad015

Nádia Eusébio, Raquel Castelo-Branco, Diana Sousa, Marco Preto, Paul D’Agostino, Tobias A. M. Gulder, Pedro N. Leão* (2022). Discovery and Heterologous Expression of Microginins from Microcystis aeruginosa LEGE 91341

ACS Synth Biol doi: 10.1021/acssynbio.2c00389

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Teresa P. Martins, Nathaniel R. Glasser, Duncan J. Kountz, Paulo Oliveira, Emily P. Balskus*, Pedro N. Leão* (2022). Biosynthesis of the Unusual Carbon Skeleton of Nocuolin A ACS Chem. Biol. 2022, 17, 2528−2537 DOI: 10.1021/acschembio.2c00464

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Sara Freitas, Raquel Castelo-Branco, Arlette Wenzel-Storjohann, Vitor M. Vasconcelos, Deniz Tasdemir, Pedro N. Leão* (2022). Structure and Biosynthesis of Desmamides A–C, Lipoglycopeptides from the Endophytic Cyanobacterium Desmonostoc muscorum LEGE 12446 J. Nat. Prod. 2022, 85, 7, 1704–1714 doi: 10.1021/acs.jnatprod.2c00162 

Adriana Rego, Antonio Fernandez-Guerra, Pedro Duarte, Phillip Assmy, Pedro Nuno Leão*, Catarina Magalhães* (2021).

Secondary metabolite biosynthetic diversity of Arctic Ocean metagenomes Microbial Genomics 7: 000731. doi: 10.1099/mgen.0.000731

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Eusebio N, Rego A, Glasser NR, Castelo-Branco R, Balskus EP*, Leao PN* (2021). Distribution and diversity of dimetal-carboxylate halogenases in cyanobacteria. BMC Genomics 22:633. doi:10.1186/s12864-021-07939-x

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Figueiredo SAC, Preto M, Moreira G, Martins TP, Abt K, Melo A, Vasconcelos VM, Leao PN (2021), Discovery of cyanobacterial natural products containing fatty acid residues. Angew Chem Int Ed 60(18):10064. doi:10.1002/anie.202015105

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Abt K, Castelo-Branco R, Leão PN (2021) Biosynthesis of Chlorinated Lactylates in Sphaerospermopsis sp. LEGE 00249. J Nat Prod 84(2):278. doi: 10.1021/acs.jnatprod.0c00950

Ribeiro I, Girão M, Alexandrino DAM, Ribeiro T, Santos C, Pereira F, Mucha AP, Urbatzka R, Leão PN, Carvalho MF (2020) Diversity and Bioactive Potential of Actinobacteria Isolated from a Coastal Marine Sediment in Northern Portugal. Microorganisms 8(11):E1691. doi: 10.3390/microorganisms8111691.

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Azevedo J, Antunes JT, Machado AM, Vasconcelos V, Leão PN*, Froufe E* (2020) Monitoring of biofouling communities in a Portuguese port using a combined morphological and metabarcoding approach. Sci Rep 10:13461. doi:10.1038/s41598-020-70307-4

Antunes JT, Sousa AG, Azevedo J, Rego A, Leão PN, Vasconcelos VM (2020). Distinct temporal succession of bacterial communities in early marine biofilms in a Portuguese Atlantic Port. Front Microbiol 11:1938. doi: 10.3389/fmicb.2020.01938

Brito Â, Vieira J, Vieira CP, Zhu T, Leão PN, Ramos V, Lu X, Vasconcelos VM, Gugger M, Tamagnini P (2020). Comparative Genomics Discloses the Uniqueness and the Biosynthetic Potential of the Marine Cyanobacterium Hyella patelloides. Front Microbiol 11:1527. doi: 10.3389/fmicb.2020.01527

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Brandão P, Moreira J, Almeida J, Nazareth N, Sampaio-Dias IE, Vasconcelos V, Martins R, Leão P, Pinto M, Saraíva L, Cidade H (2020). Norhierridin B, a New Hierridin B-Based Hydroquinone with Improved Antiproliferative Activity. Molecules 25(7):1578. doi:10.3390/molecules25071578

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Reis JPA, Figueiredo SAC, Sousa ML, Leão PN (2020). BrtB is an O-alkylating enzyme that generates fatty acid-bartoloside esters. Nat Commun 11,1458. doi:10.1038/s41467-020-15302-z

Rego A, Sousa AGG, Santos JP, Pascoal F, Canário J, Leão PN*, Magalhães C* (2020) Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica. Microorganisms 8:279. doi:10.3390/microorganisms8020279

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Martins TP, Ramos V, Hentschke GS, Castelo-Branco R, Rego A, Monteiro M, Brito Â, Tamagnini P, Cary SC, Vasconcelos V, Krienitz L, Magalhães C, Leão PN (2020) The extremophile Endolithella mcmurdoensis gen. et sp. nov. (Trebouxiophyceae, Chlorellaceae), a new Chlorella-like endolithic alga from Antarctica. J Phycol 56(1):208-216. doi:10.1111/jpy.12940

Rego A, Raio F, Martins TP, Ribeiro H, Sousa AGG, Séneca J, Baptista MS, Lee CK, Cary SC, Ramos V, Carvalho MF, Leão PN and Magalhães C (2019) Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods. Front Microbiol 10:1018. doi: 10.3389/fmicb.2019.01018

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Freitas S, Silva NG, Sousa ML, Ribeiro T, Rosa F, Leão PN, Vasconcelos V, Reis MA, and Urbatzka R (2019). Chlorophyll Derivatives from Marine Cyanobacteria with Lipid-Reducing Activities. Mar Drugs 17(4), 229. doi:10.3390/md17040229

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Girão M, Ribeiro I, Ribeiro T, Azevedo IC, Pereira F, Urbatzka R, Leão PN, Carvalho MFC (2019). Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds. Front Microbiol, 10:683. doi:10.3389/fmicb.2019.00683

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Martins TP, Rouger C, Glasser NR, Freitas S, de Fraissinette NB, Balskus EP, Tasdemir D, Leão PN (2019). Chemistry, bioactivity and biosynthesis of cyanobacterial alkylresorcinols. Nat Prod Rep. doi:10.1039/c8np00080h

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Costa M, Sampaio-Dias IE, Castelo-Branco R, Scharfenstein H, de Castro RR, Silva A, Schneider MPC, Araújo MJ, Martins R, Domingues VF, Nogueira F, Camões V, Vasconcelos VM, Leão PN (2019). Structure of Hierridin C, Synthesis of Hierridins B and C, and Evidence for Prevalent Alkylresorcinol Biosynthesis in Picocyanobacteria. J Nat Prod 82(2):393-402. doi:10.1021/acs.jnatprod.8b01038

 Martins J, Leikoski N, Wahlsten M, Azevedo J, Antunes J, Jokela J, Sivonen K, Vasconcelos V, Fewer DP, Leão PN (2018). Sphaerocyclamide, a prenylated cyanobactin from the cyanobacterium Sphaerospermopsis sp. LEGE 00249. Sci Rep 8:14537. doi:10.1038/s41598-018-32618-5

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Antunes J, Leão PN, Vasconcelos V (2018). Marine biofilms: diversity of communities and of chemical cues. Env Microbiol Rep. doi:10.1111/1758-2229.12694

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Martins TP, Reis MA, Leão PN (2018). 4-Oxo-β-apo-13-carotenone from the cyanobacterium Anabaena cylindrica PCC 7122. Chem Biodiver 15(7), e1800076. doi:https://doi.org/10.1002/cbdv.201800076

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Ramos V, Morais J, Castelo-Branco R, Pinheiro Â, Martins J, Regueiras A, Pereira AL, Lopes V, Frazão B, Gomes D, Moreira C, Costa MS, Brûle S, Faustino S, Martins R, Saker M, Osswald J, Leão PN, Vasconcelos VM (2018). Cyanobacterial diversity held in mBRCs as a biotechnological asset: the case study of the newly established LEGE Culture Collection. J Appl Phycol doi:10.1007/s10811-017-1369-y

Ribeiro T, Lemos F, Preto M, Azevedo J, Sousa ML, Leão PN, Campos A, Linder S, Vitorino R, Vasconcelos V, Urbatzka R (2017) Cytotoxicity of portoamides in human cancer cells and analysis of the molecular mechanisms of action. PLoS ONE 12(12): e0188817. doi: 10.1371/journal.pone.0188817

Dias F, Antunes JT, Ribeiro T, Azevedo J, Vasconcelos V, Leão PN (2017) Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity. Front Microbiol. doi: 10.3389/fmicb.2017.01495

Ramos VMC, Castelo-Branco R, Leão PN, Martins J, Carvalhal-Gomes S, Sobrinho da Silva F, Mendonça Filho JG, Vasconcelos VM (2017) Cyanobacterial diversity in microbial mats from the hypersaline lagoon system of Araruama, Brazil: an in-depth polyphasic study. Front Microbiol. doi: 10.3389/fmicb.2017.01233

Afonso TB, Costa MS, Rezende de Castro R, Freitas S, Silva A, Schneider MPC, Martins R, Leão PN (2016) Bartolosides E-K from a Marine Coccoid Cyanobacterium. J Nat Prod 79:2504-2513, doi:10.1021/acs.jnatprod.6b00351

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Freitas S, Martins R, Costa M, Leão PN, Vitorino R, Vasconcelos V, Urbatzka R (2016) Hierridin B isolated from a marine cyanobacterium alters VDAC1, mitochondrial activity, and cell cycle genes on HT-29 colon adenocarcinoma cells. Mar Drugs 14(8):158. doi: 10.3390/md14080158 

Leão T, Guimarães PI, de Melo AGC, Ramos RTJ, Leão PN, Silva A, Fiore MF, Schneider MPC (2016) Draft genome sequence of the N2-fixing cyanobacterium Nostoc piscinale CENA21, isolated from the Brazilian Amazon floodplain. Genome Announc 4(2):e00189-16. doi:10.1128/genomeA.00189-16

Costa MS, Rego A, Ramos V, Afonso TB, Freitas S, Preto M, Lopes V, Vasconcelos V, Magalhães C, Leão PN (2016) The conifer biomarkers dehydroabietic and abietic acids are widespread in Cyanobacteria. Sci Rep 6:23436. doi: 10.1038/srep23436

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Nimptsch J, Woelfl S, Osorio S, Valenzuela J, Moreira C, Ramos V, Castelo-Branco R, Leão PN, Vasconcelos V (2015) First record of toxins associated with cyanobacterial blooms in oligotrophic North Patagonian lakes of Chile—a genomic approach. Internat Rev Hydrobiol 101(1-2):57-68. doi:10.1002/iroh.201401780

 
Almeida JR, Freitas M, Cruz S, Leão PN, Vasconcelos V, Cunha I (2015) Acetylcholinesterase in Biofouling Species: Characterization and Mode of Action of Cyanobacteria-Derived Antifouling Agents. Toxins 7:2739-2756. doi:10.3390/toxins7082739. 

Leão PN, Nakamura H, Costa M, Pereira AR, Martins R, Vasconcelos V, Gerwick WH, Balskus EP (2015) Biosynthesis-assisted structural elucidation of the bartolosides, chlorinated aromatic glycolipids from Cyanobacteria. Angew Chem Int Ed 54(38):11063-7. doi: 10.1002/anie.201503186.

Antunes, JT, Leão PN, Vasconcelos V (2015) Cylindrospermopsis raciborskii: Distribution, phylogeography and ecophysiology of a global invasive species. Front Microbiol 6: 473. doi: 10.3389/fmicb.2015.00473.

 
Costa MS, Costa M, Ramos V, Leão PN, Barreiro A, Vasconcelos V, Martins R (2015) Picocyanobacteria from a clade of marine Cyanobium revealed bioactive potential against microalgae, bacteria and marine invertebrates. J Toxicol Env Heal A 78(7): 432-442. doi: 10.1080/15287394.2014.991466

Pereira AL, Bessa LJ, Leão PN, Vasconcelos V, Costa PM (2015) Bioactivity of Azolla aqueous and organic extracts against bacteria and fungi. Symbiosis 65(1): 17-21. doi: 10.1007/s13199-015-0316-4